Kantitatif RT-PCR (RT-qPCR) ile MikroRNA (miRNA) Ekspresyon Profillemesi
نویسندگان
چکیده
Gen ekspresyonunun post-transkripsiyonel düzenleyicisi olarak bilinen miRNA’lar ökaryotik canlılarda çeşitli fizyolojik ve patolojik süreçlerde rol oynamaktadır. miRNA'ların rollerinin ortaya konulması ile birlikte üzerine yapılan çalışmaların sayısı da gün geçtikçe artmaktadır. Yapılan çalışmalar bütün hücre doku tiplerinde eksprese olduğu konmuştur. miRNA ekspresyon profilinin konması için yöntemler öneril-mesine rağmen, yüksek duyarlılığı özgüllüğü nedeniyle RT-qPCR altın standart kabul edilmektedir. Bu derle-mede; profillemesi sürecindeki adımlar detaylı özetlenmiş konu ilgili lite-ratür bilgisine yer verilmiştir.
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ژورنال
عنوان ژورنال: Erciyes üniversitesi veteriner fakültesi dergisi
سال: 2021
ISSN: ['1304-7280', '2667-5498']
DOI: https://doi.org/10.32707/ercivet.878031